I. Overview The new generation of HH·CP carbon dioxide incubator is a high-performance carbon dioxide incubator developed by the company with many years of technical experience and the introduction of new technology. It has the characteristics of fast heating and small temperature fluctuations. It is an immunology and oncology research. The key equipment necessary for genetic and biological engineering is widely used in the research and production of medicine, microbiology, agricultural science, and pharmaceutical science. Second, the structure This machine is a bench-top carbon dioxide incubator, which consists of a sprayed plastic case, a stainless steel working chamber and an electronic control part. Carbon dioxide cylinders and carbon dioxide pressure reducing valves must be used when using. The carbon dioxide gas must be pure and the pressure relief valve pressure should be stable. Carbon dioxide cylinders are pressure vessels and are supplied by the user. The pressure reducing valve is a special pressure reducing valve for carbon dioxide, and its performance directly affects the accuracy of the carbon dioxide concentration in the tank, so it is supported by the company. 1. The machine is directly heated (air jacket type) or water tank heating (water jacket type) for the inner chamber wall, and the temperature rises quickly and the uniformity is good. Third, the main technical indicators Fourth, the method of use Five, matters needing attention Appendix 1 Appendix 2
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2. The temperature sensor is a Pt100 platinum resistor with good interchangeability.
3. The temperature control circuit uses two sensors, alarm and main control box temperature. Even if the main control circuit is out of control, the alarm circuit can still limit the temperature and the reliability is greatly improved.
4. The carbon dioxide input circuit is uniquely designed to recover quickly after opening the door (for example, when the setting is 5%, the recovery time is less than 6 minutes, and when the setting is 10%, the recovery time is less than 12 minutes). The carbon dioxide concentration is input by air and carbon dioxide. The concentration is not affected by humidity or the like.
5. The humidity is naturally evaporated.
1. Interior size:
Air jacket type: 500 × 400 × 400 (80 liters) 460 × 530 × 645 (160 liters);
Water jacket type: 500 × 400 × 400 (80 liters) 500 × 500 × 650 (160 liters);
2. Temperature control range: room temperature +5--50 ° C;
3. Temperature fluctuation: ±0.5 °C;
4. Temperature distribution uniformity: ±0.5 ° C (80 liters) ± 1 ° C (160 liters);
5. Set value: +1 °C to track the alarm and cut off the heating power;
6. Temperature recovery time: ≤ 15 minutes (at 37 ° C);
7. Carbon dioxide control range: 0-20% v/v;
8. Flow meter: air 100---1000ml/min; carbon dioxide 10---100ml/min;
9. Carbon dioxide concentration recovery time: concentration value × 1.2 minutes;
10. Power supply: 220VAC, 50Hz;
11. Power: ≤ 280W (500W) (air jacket) ≤ 500W (700W) (water jacket);
12. Dimensions:
Air sleeve type: 730 × 470 × 480 (630 × 770 × 770);
Water jacket type: 550 × 505 × 805 (700 × 605 × 955);
13. Working environment: temperature 5 ° C - 30 ° C; humidity ≤ 85% RH.
1. Install the special pressure reducing valve for carbon dioxide on the carbon dioxide cylinder, and there must be no air leakage at the joint. Connect the pressure reducing valve output joint with the carbon dioxide inlet fitting after the carbon dioxide incubator with a hose, and fasten it with a compression ring. The cylinder is not open yet.
2. Before turning on the power, first clean the inner chamber of the box with alcohol, and then use an ultraviolet sterilization lamp or an ozone sterilizer to disinfect for one hour.
3. Power on When the power is turned on, the main power switch is set to "1" and the green indicator light is on. For the water-tight (water jacketed) CO2 incubator: the first time the unit's low water level indicator is illuminated and an alarm is issued. The heater heating power is disconnected. At this time, the rubber hose should be connected to the faucet and the tank inlet port to open the faucet. The water level gradually rises after a while. When the low water level indicator is off, the alarm disappears. The faucet should be turned off after adding about 1 liter of water. If a large amount of water continues to flow, the water will overflow. Damage to the cabinet, affecting the normal operation of the unit. When the low water level indicator is high, water should be added in time, otherwise it will affect the temperature inside the box.
(1) Adjust the temperature setting to the desired temperature (for example, 37 ° C), and the heating indicator (green) should be lit to indicate that it is heating. When the required operating temperature is low, in order to prevent the initial heating, the temperature rises too quickly to cause temperature overshoot, and the secondary setting method can be adopted. If the required operating temperature is 37 ° C, set 35 ° C for the first time. When the temperature overshoot began to fall back, set it to 37 °C for the second time. This can reduce and eliminate temperature overshoot and enter the constant temperature as soon as possible.
(2) Set the carbon dioxide solenoid valve switch to “0â€. (The solenoid valve is closed, because the solenoid valve will be hot for a long time when the carbon dioxide is not cultured, which will affect the life of the solenoid valve.)
(3) After the temperature reaches the set value, the carbon dioxide cylinder is opened. (Before opening, the pressure reducing valve should be loosened as much as possible to prevent the pressure of the pressure reducing valve from being too high, causing the gas hose to burst!) The pressure gauge on the pressure reducing valve Indicate the carbon dioxide pressure inside the cylinder and slowly turn the relief valve knob clockwise. The pressure output of the pressure reducing valve is indicated as 0.05 MPa, and the pointer is in the middle of the reticle. At this time, use the dial to set the required carbon dioxide concentration (factory adjusted to 5%), turn on the carbon dioxide air intake switch, set "1", then there will be carbon dioxide into the room. As the concentration increases, the LED shows the value of the carbon dioxide concentration. When the set value is reached, the solenoid valve is cut off and switched to air and carbon dioxide. At this time, the air flow meter float should be indicated at 760ml/min (already adjusted at the factory), the carbon dioxide flow meter float should be indicated at 40ml/min (factory adjusted); if the float deviates from the above value, the adjustable needle valve can be used fine Adjust to 760ml/min (air) and 40ml/min (carbon dioxide) accurately. (When the door is quickly recovered after opening the door, there is no air flow and the carbon dioxide is less than 40 ml/min, which is normal.
(4) The factory is generally adjusted to 5% ± 0.3% carbon dioxide concentration, which can meet the requirements of the majority of users.
(5) When the temperature reaches the set value, the fluctuation is within ±0.5 °C, and the carbon dioxide concentration also meets the requirements, the cell culture can be carried out. The water tray is placed to make the humidity meet the requirements, and the natural evaporation is generally up to 95%.
(6) After using the machine for the first time or after using it for a long time, it should be operated according to the above requirements, and should be checked for contamination inside the box before formal cultivation.
4. Shutdown Before shutting down, the carbon dioxide cylinder should be closed, the carbon dioxide air inlet switch should be set to “0â€, and then the power should be cut off. If it is not used for a long time, the water tray should be taken out and the inside of the box should be wiped dry. When the carbon dioxide switch is set to “0â€, the box temperature is energized at 37 °C for two hours to ensure that the box is dry, and then the power is cut off.
1. This unit should be installed in a place where the air is clean and the temperature difference changes little, and there is no direct sunlight. There must be no strong electromagnetic fields and radiant energy around.
2. Read the instruction manual carefully before starting the machine and master the correct use method. Pay special attention to loosening the pressure reducing valve before the cylinder is opened to prevent the gas hose from blasting.
3. Needle valve can not be twisted freely. During use, the pressure output of the pressure reducing valve should be monitored as 0.05Mpa air flow 760ml/min carbon dioxide flow 40ml/min (5% carbon dioxide concentration).
4. When the cylinder pressure is less than 1Kpa, it should be replaced. When replacing the cylinder, first close the cylinder switch, loosen the pressure reducing valve screw shaft, and then remove the pressure reducing valve and reinstall it on the full cylinder.
5. Open the glass door and open it. After about half a minute, close the door and clear the residual gas to prevent carbon dioxide from accumulating and overshooting when carbon dioxide is restored.
6. The components in the box side control box have been precisely adjusted. Do not adjust the adjustable part arbitrarily to avoid affecting the accuracy.
7. The incubator power supply must be well grounded for safety.
8. If there is any fault, please contact Shanghai Boxun Industrial Co., Ltd. and Shanghai Boxun Medical Equipment Factory immediately.
Sixth, the control panel schematic
l If the upper row shows over, the sensor is open or the input signal exceeds the measurement range.
l Set value and alarm value change mode button (4), the upper row displays the SP symbol, press â–² or â–¼ key to make the lower row display the required value, and then press the meter to return to the standard mode.
â— Control parameter change mode button for more than 4 seconds, the upper display shows the prompt of the control parameter, press â–² or â–¼ key to make the lower row display the required value. Continue to press the button, the parameter prompts appear in the upper row, press â–² or â–¼ key to make each control parameter the desired value, and then press the button for more than 4 seconds to return to the standard mode. (Automatically returns to standard mode after 1 minute without key press)
l Each function parameter:
Prompt name setting range initial value
RE time ratio -99-100 °C 0
Rt process value offset -99-100°C 0
DF insensitive zone 0.4-100 ° C 0.4
AL alarm point-1999-1999°C 1
T proportional band 1-100s 20
P proportional band 1-300 ° C 3
I Integration time 1-3600s 200
D Derivative time 1-3600s 50
Lc password lock 0-2 0
Ct alarm delay 0-3600s 0
l Self-tuning function of the meter After starting this function, it can automatically search according to the control object and simulate the expert operation, and set the optimal parameters to achieve the best temperature control effect.
After pressing the â–² button for 8 seconds, the AT light flashes and the meter starts to self-tune. After the auto-tuning is finished, the AT light is off, and a set of PID parameters with faster heating is obtained. The meter is controlled according to the new PID parameters.
After pressing the â–¼ button for 8 seconds, the AT light flashes and the meter starts to self-tune. After the auto-tuning is finished, the AT light is off, and a set of PID parameters that can overcome the over-temperature is obtained. The meter is controlled according to the new PID parameters.
u The new PID parameters can be checked on the meter.
u In the auto-tuning process, press the â–² or â–¼ button for 8 seconds and the AT light is off. The auto-tuning is aborted and the meter is controlled according to the original PID parameters.
l Detailed description of each parameter:
rE—time ratio control instrument reset (RESET), because the heating power of the device is different, the controlled temperature is different, so that the proportional control instrument generates a static difference, which is overcome by adjusting this value (in the object) The load is unchanged under the premise).
For example, the set temperature of a device is 200 ° C, the temperature after the system is stable is 204.5 ° C, indicating that the static difference is 4.5 ° C, at this time rE is set to –4.5 ° C, after a period of control, the temperature will be stable at 200 Near °C.
Rt—Measurement value correction, because the sensor and the instrument itself are limited by the accuracy, the temperature measurement produces an error, which is adjusted by adjusting this value.
For example, the set temperature of a meter is 200 ° C, the measurement temperature is also 200 ° C, measured with a high-precision mercury thermometer is 201.7 ° C, at this time rt is set to 1.7 ° C, and the meter display is also 201.7 ° C. After a period of control, the temperature will remain stable at 200 ° C and the same temperature as the high-precision mercury thermometer.
dF—Position control and insensitive zone setting for alarm control. If dF is set to 1 °C, the meter will change the output at ±0.5 °C of the control point and alarm point.
AL—Setting the alarm control point.
When the meter is a deviation alarm, the actual alarm control point is: SP+AL. For example, the set temperature SP of a meter is 200.0 °C, the AL of the alarm control point is 10.0 °C, and the actual alarm control point should be: 200.0+10.0=210.0 °C, that is, higher than SP+AL+1/2dF alarm The clutch is closed and released below SP+AL+1/2dF. Another example: the alarm control point setting AL is –10.0°C, the actual alarm control point should be: 200.0-10.0=190.0°C, and the output state also changes, that is, lower than the SP+AL-1/2dF alarm relay. The device is sucked and released above SP+AL-1/2dF.
P—proportional band setting. When the temperature fluctuates regularly (system oscillation), the proportional band should be increased. When the temperature drifts irregularly, the proportional band should be reduced.
I—Integral time setting, when the temperature fluctuates regularly (system oscillation), the integration time should be increased. When the temperature cannot be eliminated for a long time, the integration time should be reduced.
d—Derivative time setting, the increase of the differential time helps to reduce the over temperature of the system.
T-control cycle setting, generally speaking, the control period with AC contactor considers its mechanical life to be between 20 seconds and 60 seconds. The control cycle with solid state relay and thyristor switch should be controlled in 3 seconds -10 Between seconds.
Lc—lock control, 0: all parameters can be modified; 1: only the given value (SP) can be modified; 2: all parameters cannot be modified.
Ct—Alarm delay time. Under normal circumstances, Ct should be set to 0.
In-tank pollution inspection: use two culture dishes with incubator, one culture dish is artificially polluted, such as puffing on the surface of the culture dish, and then capped and placed in the box for cultivation: another control, no external pollution, half-start The lid is placed in a box for cultivation. The above-mentioned contaminated bacteria should have the growth of bacteria, and the control should be free of bacteria, which proves that the box has been disinfected and cleaned, and the incoming gas is not contaminated. At this time, it is put into training and use.
The carbon dioxide concentration is calculated by the following formula: air flow and supplemental air flow:
Carbon dioxide concentration = carbon dioxide flow / min ÷ (carbon dioxide flow / min + air flow / min)
example:
5% air 760ml/min carbon dioxide 40ml/min
10% air 720ml/min carbon dioxide 80ml/min
15% air 510ml/min carbon dioxide 90ml/min
20% air 400ml/min carbon dioxide 100ml/min 