Cell migration is the movement of a cell after it receives a migration signal or senses a concentration gradient of certain substances. During the process of cell migration, the cells repeatedly repeat the process of extending the protruding body to the front and then pulling the cell body. In tumor cell behavioral analysis, cell invasion is a prelude to tumor cell metastasis, and metastasis is the continuation and outcome of tumor cell invasion. Cell migration requires the coordination of internal and external factors. External factors refer to extracellular signaling molecules (including chemokines), internal factors refer to the cell's signaling system and the cytoskeleton and molecular motors that perform motion, as well as various molecules involved in the formation of focal adhesions.
The current conventional method is to perform cell behavior analysis in the upper and lower chambers of the invasion chamber containing a carbonate filter. If you only need to analyze the cell's ability to exercise or need to understand the effect of the drug on cell motility, directly put the filter in the upper and lower chambers of the invasion chamber, which is also used as a traditional cell migration analysis model; if you need to infiltrate the cells For the analysis, it is necessary to lay the extracellular matrix component on the filter membrane to ensure that the membrane pores are uniformly covered by the matrix protein, the cells cannot pass freely, the hydrolase must be secreted, and the substrate can be passed through the deformation movement. Protein filter. Finally, the transmembrane cells are fixed, stained and counted to obtain the invasive ability of the cells.
The difference between cell migration assay and cell invasion assay is mainly whether the membrane protein is coated on the membrane. However, the traditional method requires complicated reagent preparation and optimization of the experimental process, and finally the cumbersome manual counting also affects the validity of the experimental data. Amy Jie Technology Co., Ltd. recommends Trevigen's full range of Cultre® cell behavior analysis protocols to provide complete tumor cell behavior analysis including cell invasion, cell migration, cell adhesion, in vitro vascular regeneration, and vascular permeability analysis. Products and kits.
The Cultrex® Cell Migration Assay Kit provides all the necessary reagents and migration chambers for cell migration analysis. The analysis kit finally uses Calcein-AM fluorescent dye to achieve the quantification of invasive cells, ensuring extremely high precision, and thus obtaining the best migration experiment results. The Cultrex® Cell Migration Assay Kit provides components such as cell migration chambers, cell eluates, cell separation fluids, and the fluorescent dye Calcein-AM.
| Product name | Brand | Item number | product manual |
| Cultrex® 24 Well Cell Migration Assay | Trevigen | 3465-024-K | 24 fluorescence analysis |
| Cultrex® 96 Well Cell Migration Assay | Trevigen | 3465-096-K | 96 fluorescence analysis |
Trevigen is a fast-growing US biotechnology company focused on oncology research products and services for cell-wide assays, apoptosis, DNA damage and repair, tumor cell function and behavior. As the general agent of Trevigen in China, Amy Jie and Trevigen provide Chinese researchers with the best and latest quality products and technical services in the fields of oxidative stress, cell damage and tumor cell behavior research.
Active Pharmaceutical Ingredient (API)
Active Pharmaceutical Ingredient (API)
We are a manufacturer and supplier of raw materials, intermediates for pharmaceuticals, as well as Active Pharmaceutical Ingredient (API). Our focus is on providing customers with high quality, fair priced products, coupled with our broad range of technical know-hows on fundamentals, vast industrial experience on applications, professional expertise on health and safety, and efficient logistics on customer services. We have established good market for our intermediates in India, Korea and EU. Besides of their intermediates, we can also synthetize the API directly. We also have some API by fermentation, Polymyxin B Sulfate is one of them and we are developing more APIs as well.
Our production is based on GMP standard workshop, according to international Pharmacopoeia standard, making sure your experience with Sunshine Biotech is always enriching, satisfying and fulfilling.
Active Pharmaceutical Ingredient,Polymyxin Sulfate,Ziprasidone Hcl,Polymyxin Sulphate
Nanjing Sunshine Biotech Co., Ltd , https://www.sunshine-bio.com