Significant progress has been made in the technological innovation of rapid propagation of forest grass and flowers in China

Under the support of the National 863 Program, China has made important progress in technological innovation of rapid propagation of flowers and plants in China, providing guarantees for the scale production and industrial development of new varieties.

Large-scale cutting propagation technology: The technology of “producing seed production and asexual use” of conifers is the key link in the cultivation of timber forests. Through the growth regulation, environment and facilities technology, the survival rate of asexual reproduction of hard-to-pure root species has been greatly improved, and the survival rate of cuttings such as larch and spruce has reached 85% to 95%; and the improvement of larch seedlings has been improved. The garden technology has broken through the difficulties in the low rooting rate of coniferous tree cuttings and realized the scale propagation of coniferous species clones and the variability of afforestation clones.

Tissue culture technology: breakthrough teak and papaya induced rooting technology, systematic optimization of teak, papaya rapid propagation technology system, seedlings proliferation stability and reproduction rate increased significantly; systematic research of tea, osmanthus and other Chinese characteristics of the flower Rapid propagation technology has successfully established the rapid propagation system of Osmanthus fragrans, and the embryo culture has released the dormancy characteristics of the seeds, so that the seeds can germinate without undergoing physiological postharvest; the seven kinds of embryonic cotyledons of cold-tolerant, aromatic camellia and tea pear are broken through Somatic embryogenesis and regeneration technology; establishment of broccoli embryo culture and embryoid body induction and culture technology system, successful access to embryo culture seedlings and embryoid body seedlings, in order to speed up the peony breeding cycle, improve breeding efficiency, reduce breeding costs Has opened up a new way; improved the tissue culture techniques of waxy, peony, phalaenopsis, Begonia, Cymbidium, Camellia and other species, optimized the cultivation conditions; established Phalaenopsis, Chinese name orchid, sweet-scented osmanthus, camellia , Pinellia, Cherry, and Anthurium tissue culture rapid propagation technology and its technological process, successfully established a new breed of breeding and propagation system, increased the number of The rate of colonization decreased the mutation rate, effectively improved the quality and reduced the cost. The Mengnong hybrid wheatgrass mature embryo tissue culture regeneration system and the Zoysia grass regeneration system were established, and the factory-scale large-scale varieties of forest grass and flowers were realized. Production has played an important role in accelerating the progress of afforestation and flower industry in China.

Somatic Embryogenesis and Scale Reproduction: Breakthroughs in induction, fixation, and proliferation of embryogenic cells such as larch, Pinus bungeana, Pinus armandii, North American pine, green bar, Haloxylon ammodendron, and Pinus massoniana, and synchronization control techniques, somatic cells Embryogenesis and rooting techniques have been used to construct a coniferous tree engineering breeding and breeding technology platform. For the first time in China, a system of coniferous embryonic cell lines has been systematically established. For the first time, a unique bioreactor and its control system suitable for liquid-scale proliferation of embryogenic cells of larch has been obtained. 2.5-liter and 20-liter larch-specific organisms have been developed. The reactor broke through the proliferation of different embryonic cell lines under liquid culture conditions, and the synchronous growth of somatic embryos reached 85%. The cryogenic, resuscitation, and thawing of cell line cryopreserved cells were improved. The Weyerhaeuser Company has fully affirmed the key links of the embryonic cellogenesis technology of conifers and the scale-up technology of bioreactors, and established a cooperative relationship.

The technology system of embryogenesis and optimization regulation of saffron was studied and established. The proliferation coefficient and differentiation ability of saffron-embryonic cells were improved, an efficient and stable regeneration system was established, and propagation coefficient, biomass, and budding rate were improved.