U.S. frogs are bred according to conventional methods and it is difficult to achieve a market size of more than 250 grams in the current year. To shorten the frog breeding cycle, the following measures can be taken.
1. Artificial indoor temperature-controlled culture is generally conducted in winter. Choose a thick, well-sealed, well-sized room for the frog house and a frog pool with a concrete structure. The area of ​​the pool is generally no more than 4 square meters, the pool is 0.8 meters high, the bottom is inclined at 15 degrees, and the pool can be counted side by side, each with an inlet and outlet. Before restocking, use a solution of 3ppm bleach (containing about 33% of available chlorine) to disinfect the pool, frog house and utensils, etc. After 72 hours, drain the pool water. The healthy, disease-free frogs were sterilized with 15 ppm potassium permanganate solution and 3% saline for 15 minutes and then stocked. Usually fed by artificial feed pellet feed, the daily feeding amount is 10% to 15% of the frog's weight. The room temperature is controlled above 20°C. Rinse once daily with water every morning and evening. Disinfect with 1ppm bleach or potassium permanganate once every two weeks. Regularly mix 100 kg of feed with 20 grams of oxytetracycline for 2 days. This method can make American frogs grow normally in winter, and the survival rate is as high as 95%. The disadvantage is that the investment is large.
2. Cultivation of greenhouses usually takes place during autumn and winter and late winter. Above the frog pool, take a 1.5m high plastic greenhouse. Pool width of 4 to 5 meters, a small pool in the middle for easy operation. Daily management work is basically the same as conventional farming. Before and after the winter, greenhouses were used for cultivation to extend the frog's feeding time by about 2 months. The young frogs in the autumn and winter seasons could fully ingest before wintering, which was beneficial to increase the survival rate in winter; the frog breeding in the late winter was conducted in greenhouse cultivation in advance. , May spawning 15 to 30 days in advance.
3. Artificial oxytocin selection Healthy second instar frogs with relatively long ancestry, long form, and obvious sexual characteristics as breeding frogs generally require a frog weighing more than 400 grams. Chorionic gonadotropin (HCG) and luteinizing hormone-releasing hormone analogue (LRH-A) are commonly used for artificial labor. More than 500 grams of female frogs, injected HCG2000 units plus LRH-A 200 micrograms of mixed drugs 1.5 to 2 milliliters, females weighing less than 500 grams of 1-5 milliliters of injection, male frog dose halved. At the 45-degree angle of the gluteal muscles, a 1.5-cm needle was injected. After the injection, the injection site was swabbed with an alcohol swab and carefully placed into the spawning pool at a 1:1 male-male ratio. After 40 hours, the frog hugs to spawn. This method can advance the spawning period, spawning at the same time, and facilitate daily management.
4. Pulse electrostimulation of early embryos Two hours after spawning, the fertilized eggs were moved into trays, treated with electrical pulses for 6 minutes, and then transferred to cages for hatching. In this way, the hatching rate can be increased by more than 10%, the worms can be metamorphosis 7-10 days earlier, and the frog's disease resistance can be enhanced and the growth rate can be increased.
5. Embed the cages in the earthen pond to hatch and raise the earthworms to adjust the water level of the earthen pond to about 50 cm. Incubation cages are 1 meter long and 0.7 meters high. The bottom of the cage and the bottom of the cage are fixed with wooden stakes and the water is 30 centimeters. The fertilized eggs should be evenly distributed in the incubator and be floating. At the same time, attention should be paid to the timely removal of dead eggs. The hatching density should be less than 10,000 grains per square meter of water. Pool disinfection with earthen pond farming. Dianchi cages are usually filled with 40 cm of water, and the feeding amount is based on two hours of basic food. During the wintering period, the cages sink to the bottom of the pool.
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