Detoxification Technology of Edible Fungi Strains

In recent years, there have been many diseases of edible fungi. So far, no effective and special anti-drug drugs have been used in production. The application of plant detoxification technology to the production of edible fungi can effectively suppress the occurrence of diseases.

The so-called detoxification of plants is the separation of the tip tissue of the shoot tip of the plant, and the isolation of the tissue is carried out in a specific culture medium to obtain new seedlings of de-mycoplasma viruses and germs. Specific to the detoxification of edible fungi, strictly speaking, general tissue separation can not achieve the purpose of detoxification of bacteria species, this is because the fruiting body has grown to the middle and late stage, the probability of carrying viruses, bacteria is quite high, so The detoxification effect is not obvious. Detoxification of the tip of the mushroom growth point is ideal.

There are two specific methods for detoxification:

1. Mycelial tip detoxification technology

Use dishes that are 90 mm or 110 mm in size. In the absence of such a petri dish, a large test tube with a size of 25 mm and 200 mm can also be used, but the operation is not easy. The mother culture medium was first prepared conventionally, and was filled into a flask, sealed with a tampon, and covered with a paper sheet. At the same time, the culture dish was washed and wrapped in kraft paper. Both are sterilized at the same time. Sterilize at 121°C for 30 minutes. When the pressure in the pot to be sterilized is zero, the sterilized product is taken out and placed in a pre-sterilized inoculation box. Open the kraft envelope, hold the petri dish in one hand, and hold the flask in one hand. Adjust the lid of the petri dish by about 1 to 2 cm using a finger and pour it into the medium liquid approximately 10 to 20 ml. Place the petri dish flat and cool it into a smooth surface, commonly known as a flat plate. When the plate is cooled to 30°C or less, the strain to be detoxified is to be accessed. Inoculation method: Pick a seed source of rice size and access the edge of the plate. After inoculation, cultivating at the specified temperature (depending on the variety control temperature). When the mycelium sprouted to a maximum length of 2 cm, the tip of the mycelium was cut approximately 1 mm with a sharp-edged inoculation tool and was inserted into a fresh plate. This is 1 detoxification. Detoxification can generally be continuous 3 to 4 times. The more virus-free times, the more assured the detoxification effect.

2. The original organization of virus-free technology

This technology removes the drawbacks of the original fruit tissue being separated in the middle and late stages of growth when the original tissue is separated, and is highly likely to carry viruses and germs, thus realizing the goal of detoxification of the tip tissue. The specific operation is as follows: The base material is conventionally prepared and the ratio of nitrogen to nitrogen is 30:1. The ratio of carbon to nitrogen can not be less than 25:1, so as to prevent the hyphae from excessively growing and growing into sclerotin, and it is not easy to present the primordia. Cans of large cans are washed and ready for use. Prepare several 11cm 11cm cotton gauze. When filling 1 cm below the bottle mouth, apply a layer of gauze from the mouth of the bottle and use a flat-blade screwdriver to insert the edge of the gauze under the bottle so that it is close to the bottle. Seal sterilization, inoculation, culture and other operations are performed as usual. After the hyphae have been filled with a full bottle, temperature, humidity, light and other irritations are applied, and the primordium usually appears in about 7 days. At this time, the original form is white. When the primordium is up to 1 cm, detoxification can be performed. After the bacteria bottles were scrubbed with 75% alcohol, they were transferred to a sterilized inoculation box and no conventional fumigation was performed in the box. At the same time prepare the inoculation needle (or inoculation fishing), a plurality of blades (for alternate use), together with the bacteria bottle into the inoculation box, sprayed benzalkonium to ensure aseptic operation. Both hands were scrubbed with 75% alcohol, extended into the inoculation box, and the blades were hand-cooled after being sterilized by burning. Open the seal of the bottle and use both hands to remove the primordium with a sterile tweezers. Because the material surface is covered with a layer of gauze, the base material will not be taken out and the operation is convenient. The base of the primordium was shaved about 1 mm with a razor blade, and then the primordium was excised with a sharp-edged blade so that the size of each block was about 1 mm2. Use an inoculation needle to transfer the separated primordial plate into a plate or a large tube for routine culture. Operation points: First, when the blade is used for cutting and cutting, the blade must be replaced every time one blade is cut; second, the separation block must be placed on the edge when it is connected, and it can be connected to the edge of the plate or the front of the test tube. Generally, it is not centered. .