Progress in genome research of important crops such as rice in the field of 863 plan biology and modern agricultural technology

Following the completion of indica whole genome sequencing and indica chromosome 4 sequencing independently, the results were published in "Science" and "Nature," and disease resistance (bacterial blight, rice blast), salt tolerance, drought resistance, efficient use of nitrogen and phosphorus, A number of clones of important genes with potential application value, such as tillers, crisp stems, stem elongation, and adventitious root growth, have achieved breakthroughs. The Institute of Genetics and Development of the Chinese Academy of Sciences cooperated with the China National Rice Research Institute and other institutes. Using map-based cloning to clone and control the MOC1 gene was the first time in China in the past 20 years to clone functional genes with important agronomic shapes of main crops with independent intellectual property rights. The successful development of the world's first rice genome-wide chip contains information on 55,791 unique genes. The genome-wide microarray was used to study the gene expression profiles of rice seedlings, seedlings at the tillering stage, roots, heading stage, and ear stages at the filling stage, and the expression characteristics of different types of genes in different organs were analyzed in detail. Arabidopsis genomes have homologous and specific genes expressed in related organs. At the same time, a total of 180,000 individual T-DNA-introduced rice regenerated strains have been obtained, with mutant strains of plant type, fertility, growth stage, tiller, plant height, resistance to pests and diseases, and stress resistance; initial establishment of rice mutants The database; large-scale selection of T1 mutant families, obtained a batch of morphological mutations and drought-resistant, disease-resistant mutants; completed 10,000 T-DNA inserted flanking sequence sequencing work. The establishment and improvement of research platforms such as mutant libraries and rice genome-wide chips have laid a good foundation for the functional gene research of rice and other crops in China and molecular design of rice. Transcription factors related to epidermis hair differentiation and elongation were isolated from cotton and verified by the transgenic plants that they can initiate the initiation and elongation of epidermal hairs on the seeds of plants, and applied for these two genes and tables. The coat-specific promoter is patented. This work has made China's cotton molecular biology research work ahead of the world. These two genes are expected to obtain transgenic cotton lines with improved cotton fiber yield and quality during the fifteenth period.

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