Comprehensive prevention and control of swine erysipelas

Swine ampoules are commonly known as “hot prints.” Diamonds, rounds, and square purple rash patches appear in pig ears, back, buttocks, etc. at the time of onset. The disease is an acute fever and septicemia. It is a great hazard to the pig industry.

Swine erysipelas is caused by the bacterium of the genus Erysiphe, and it has obvious seasonality. It occurs frequently in summer and autumn, especially after the sweltering weather. The age of the affected pigs was mostly 9 months after weaning, but there were multiple pigs aged 4 to 6 months. Most of the pigs showed: the body temperature rose to 41.5 ~ 42 °C, do not eat, thirsty, hi lying, shaking, dry stool, erythema on the skin, finger pressure does not fade and other symptoms.

First, preventive measures

1. Strengthen feeding management

Pay attention to ventilation and stocking density.

2. Preventive injection

The piglet was injected with erysipelas lyophilized seedlings and diluted with aluminum gel saline. One pig per pig was injected. After 7 days, immunity was generated. At 3 months of age, pig triple vaccine was used to reinforce 1 pig per pig.

Second, the treatment

1. Antibiotic treatment

The strain of erysipelas is extremely sensitive to penicillin, and it is better treated with penicillin. The dosage is calculated to be 4000 to 5000 units per kilogram body weight of penicillin (200-250 thousand units for pigs weighing 50 kg) and injected once every 8 to 12 hours.

2. Sulfonamide therapy

Can be intravenous or intramuscular injection of 10% sodium sulfathiazole 20 ~ 40 ml, 1 or 2 times a day.

3. Chinese medicine therapy

Prescription for the ground scattered: 30 grams of earthworm, 30 grams of gypsum, 30 grams of rhubarb, Scrophularia 15 grams, 15 mg of Anemarrhena, Forsythia 15 grams, boiled with water, two pigs, each dose of 2 to 3 Secondary filling service.

Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay, that consists of a simple prepare and read method, without the requirement of separation or washing steps.

The basis of the assay is fluorescence anisotropy, also known as fluorescence polarization. If a fluorescent molecule is stationary and exposed to plane-polarized light, it will become excited and consequently emit radiation back to the polarized-plane. However, if the excited fluorescent molecule is in motion (rotational or translational) during the fluorescent lifetime, it will emit light in a different direction than the excitation plane. The fluorescent lifetime is the amount of time between the absorption moment and the fluorescent emission moment.

Typically, the rate at which a molecule rotates is indicative of its size.[1] When a fluorescent-labelled molecule (tracer) binds to another molecule the rotational motion will change, resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization.[2] Fluorescence polarization immunoassays employ a fluorophore bound antigen that when bound to the antibody of interest, will increase fluorescence polarization. The change in polarization is proportional to the amount of antigen in sample, and is measured by a fluorescence polarization analyzer.[3]